Description
For the quantitative determination of human anti-COVID-19 nucleocapsid protein ELISA (IgG class antibodies) in serum.
Introduction: Since December 2019, outbreak of COVID-19 infection has become a major epidemic threat in China and brought back the attention of pathogenic coronavirus to the spotlight. Nucleocapsid protein (NP) is the most abundant protein on the helical nucleocapsid of coronaviruses, which envelopes the entire genomic RNA. NP also interacts with other viron structural proteins to play important roles during host cell entry and virus particle assembly and release.
Human anti-COVID-19 nucleocapsid protein (NP) IgG ELISA kit is a highly sensitive and specific ELISA kit developed by ImmunoDiagnostics for the precision detection and quantitative measurement of titres of anti-NP IgG class antibodies in human serum.
Assay Principle: Recombinant nucleocapsid protein (NP) of COVID-19 (Cat No. 41A220) coated onto the plate wells can specifically recognize anti-NP antibodies in human sera. After a 1 hour incubation, anti-NP antibodies are captured by immobilized NP protein while the unbound components were discarded and washed away. Afterwards, HRP-conjugated anti-human IgG is added for another 1 hour incubation, wherein the anti-human IgG detects IgG class antibodies previously bound to NP protein on the plate. After removal of nonspecific bindings, bound anti-human IgG is revealed by the addition of substrate solution containing 3,3?,5,5?-Tetramethylbenzidine (TMB), resulting in the formation of a blue color. Color reaction will be further stopped by 2M H2SO4, transforming the blue color to yellow signals. The absorbance of yellow reaction mixture is measured by plate reader at 450nm. The higher the reading is, the higher concentration of anti-NP IgGs